中国科学院大学 中国科学院大学新闻网

报告摘要：

Long-lived memory B cells (B_Mem) are an archive of past antibody (Ab) responses that persists for much of the host lifespan. By contrast, circulating antibodies typically decline once the immunogen is cleared. This raises the possibility that the B_Mem pool might provide a more accurate picture of discrete antibody specificities that arise and decline during the course of an antibody response. This could be especially important for correlating antibody specificity with protective immunity in infectious diseases such as AIDS. By studying Ab and B_Mem of HIV-1 infected individuals who naturally suppress HIV-1 without therapy (NVS), we found high frequencies of B_Mem specific for CD4 induced (CD4i) or CD4 binding site (CD4bs) epitopes of gp120 that were discordant with contemporaneous plasma antibody responses to these epitopes. These data suggest that plasma Ab responses can underestimate the breadth of humoral immunity and that analyses of B_Mem should be included in studies correlating antibody specificity with protective immunity to HIV-1. A novel technique for rapid selectively cloning human monoclonal antibody (mAb) from B_Mem was developed to study the Ab responses in NVS and 50 novel human mAbs against HIV-1 gp120 were obtained.

 

主讲人简介：

Education & Employment History

2007 - present  Faculty Research Associate, 2009 Assistant Professor, Institute of Human Virology, University of Maryland School of Medicine

2004 – 2007    Faculty Research Associate, Institute of Human Virology, UMBI, Baltimore, MD, USA

2001 - 2004    Post-Doctoral Research Associate, Dept. of Microbiology, Mount Sinai Hospital, Toronto, ON, Canada

1994 – 1998    Research Associate, Lab. of Tumor Viruses and AIDS, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China

1998 – 2001  Postdoctoral Fellow, McGill University AIDS Center, Lady Davis Institute-Jewish General Hospital, Montreal, QC, Canada

1998   Ph.D. in Immunology, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China

1989  B. S. in Bioengineering, 1993 M. S. in Molecular Biology,Dept. of Biology, Nankai University, Tianjin, China